Bedbugs can serve as reservoir for Chlamydophila pneumoniae

   According to investigators at the University of Central Missouri at Warrensburg, adult Cimex lectularius (bedbugs) can serve as a reservoir for Chlamydophila pneumoniae and may possibly contribute to the spread of the pathogen. They presented their study as a poster at ASM 2013, held in May 2013 in Denver.

   The investigators acquired three insect colonies from different pest control companies, placed them in 95% ethanol, then isolated DNA from frass, ingestively na├»ve nymphs, fed nymphs, and adults. Primers were selected for C. pneumoniae based off published sequences. PCR was performed using a 51oC annealing temperature and visualized as a 696 bp band via ethidium bromide-laden agarose gels run for 1 hour at 80 volts. Bands were excised, purified via enzyme digestion, and sequenced.

  According to the University of Central Missouri researchers, the results showed bands of C. pneumoniae in 78% of fed adult populations. However, all nymphs and frass were negative for C. pneumoniae. PCR was performed at least five times on each sample to ensure no false results. "It appears that adult Cimex lectularius can serve as a reservoir for C. pneumoniae and may possibly contribute to the spread of the pathogen," the researchers concluded.